EFFECT OF CAFFEIC ACID LOADED BIOPOLYMERS ON CORTISOL AND KIDNEY AGAINST HIGH FAT DIET INDUCED METABOLIC SYNDROME IN ALBINO MICE

Abstract

The current study aimed to assess the impact of biopolymers loaded with caffeic acid on cortisol of albino mouse models of experimentally induced metabolic syndrome (MetS).48 healthy adult albino mice were randomly assigned to 8 equal groups. For 4 weeks, the "Control group," Group 1, was fed a typical chow diet. For 35 days, caffeic acid-loaded biopolymers (300 mg/kg) were administered to Groups 3, 4, and 5 (the control group).MetS was initially induced in Group 2 (MetS), Groups 6, 7, and 8 (MetS + Caffeic Acid Loaded Biopolymers) by an oral dosage of 30% fructose and a high-fat diet for 4-6 weeks. After that, Group 2 continued to consume a high-fat, high-fructose diet for 35 days, whereas Groups 6, 7, and 8 got biopolymers enriched with caffeic acid for the same amount of time. Blood samples from each animal were taken on days 15th and 35th. The centrifuged samples were then stored at -40°C to estimate biochemical and hormonal parameters. According to findings, MetS highly significantly raised levels of triglycerides, cholesterol, LDL, creatinine, urea, and cortisol while non significantly increased albumin, highly significantly lowered levels of total protein and HDL, and non-significantly decreased levels of globulins. Contrarily, delivery of caffeic acid ladened with biopolymers led to the maintenance of all parameters' normal levels. After caffeic acid loaded biopolymers were administered to the MetS group, there was a decrease in the levels of triglycerides, albumin, cholesterol, LDL, creatinine, urea, and cortisol and an increase in the levels of total protein, HDL, and globulins. Accordingly, it can be said that caffeic acid loaded biopolymers are a promising drug with the potential to ameliorate MetS morbidities on several biochemical parameters based on the findings of the current investigation.