GREEN SYNTHESIS OF SeNPs FROM AMARANTHUS VIRIDIS AND DATURA ALBA AND THEIR BIOLOGICAL ACTIVITIES

Abstract

This research focus on the eco-friendly production of Selenium Nanoparticles (SeNPs) using methanolic leaf extracts obtained from Amaranthus viridis L. and Datura alba L. The bio-synthesized Se-NPs have been analyzed by the UV-Visible spectroscopy, Fourier Transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and SEM (Scanning Electron Microscopy). The Ultraviolet-Visible Spectroscopy results displayed clear absorption peak of Selenium nanoparticles (SeNPs) at 280 nm for A. viridis and at 302 nm for D. alba. Both of the test plants displayed –OH and –CH₃ groups, in the FTIR spectra. SEM images revealed small, largely rounded SeNP's from A. viridis and D. alba, whereas XRD analysis demonstrated the crystalline character of the Selenium nanoparticles. According to the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical assay, D. alba showed higher antiradical potential in comparison to A. viridis. The antimicrobial activity of SeNPs green synthesized from A. viridis and D. alba was checked using Penecillium verrucosum Dierckx. as test fungus. Different applied concentrations (0.5%, 1%, 1.5%, 2% and 2.5%) of green synthesized Selenium nanoparticles (SeNPs) significantly reduced fungal growth of P. verrucosum as compared to control treatment. 2.5% concentration of SeNPs from both A. viridis and D. alba showed maximum reduction in fungal biomass at 65%. SeNPs derived from D. alba showed slightly higher reduction as compared to A. viridis mediated SeNPs. From this study it can be concluded that Selenium nanoparticles synthesized from A. viridis and D. alba possess strong antioxidant and antimicrobial potential which can be utilize further in biomedicine, pharmacology, and sustainable agriculture. Using green synthesized SeNPs as bio fungicide can decrease the hazard of environmental pollution linked with chemical fungicide.